A TaqManR RT-PCR Assay for the Detection of Feline calicivirus
نویسندگان
چکیده
منابع مشابه
Development of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus
Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
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Background: Human rotavirus (HRV) is the causative agent of severe gastroenteritis in children and responsible for two million hospitalizations and more than a half-million deaths annually. Sequence characteristics of the gene segments encoding the VP7 and VP4 proteins are used for the genotype classification of rotavirus. A wide variety of molecular methods are available, mainly based on rever...
متن کاملComparative analysis of different TaqMan real-time RT-PCR assays for the detection of swine Hepatitis E virus and integration of Feline calicivirus as internal control.
AIMS The aim of this study was to compare the performance of four TaqMan RT-PCR assays with a commonly used nested RT-PCR and to include the Feline calicivirus (FCV) as an internal control. METHODS AND RESULTS RNA extracted from 87 swine faecal samples and 103 swine blood samples was subjected to different detection systems. Faecal samples naturally contaminated with Hepatitis E virus (HEV) a...
متن کاملDevelopment of a Multiplex RT-PCR Assay for Detection of the Causal Agents of Citrus Tristeza and Cachexia Diseases with Coamplification of Plant mRNA as an Internal Control
Background and Aims: Plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. Reverse transcriptase-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certification of plant propagating materials. The main object of the present study was the optimization ...
متن کاملdetermination of feline calicivirus in cats in ahvaz district, southwest of iran by rt-pcr (a preliminary study)
feline calicivirus (fcv) is a highly infectious respiratory pathogen of domestic cats with a widespread distribution. in order to assess how fcv circulates in feral and household cats, we have carried out the first study on the fcv detection in ahvaz district from december 2008 to november 2009. oropharyngeal, nasal and ocular swabs of one hundred cats (70 feral and 30 household) were evaluated...
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ژورنال
عنوان ژورنال: International Journal of Virology
سال: 2007
ISSN: 1816-4900
DOI: 10.3923/ijv.2007.100.106